Radioimmunoassay for Luteinizing Hormone in Bovine Species Based on Commercially Available Reagents
نویسندگان
چکیده
منابع مشابه
Parathyroid hormone assay in primary hyperparathyroidism: experiences with a radioimmunoassay based on commercially available reagents.
A radioimmunoassay is described for parathyroid hormone in human serum, in which commercially available reagents are used almost exclusively. This assay can be done by any laboratory with experience in radloimmunoassay. Thirty-two of thirty-three patients with surgically proven primary hyperparathyroidism had detectable concentrations of parathyroid hormone in their serum, and concentrations of...
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We describe a sequential double-antibody assay for measuring C-terminal parathyrin in serum with commercially available reagents. Intact bovine hormone, used as a working standard, is iodinated by conventional Chloramine T procedures. The antibody affinity is characteristic of high affinity binding (1.4 to 1.6 x 10(10) L/mol of intact parathyrin). The antibody also cross reacts with a C-termina...
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A clincally useful and reproducible radioimmunoassay for human homologous prolactin, established with commercially available reagents, was studied and validated. We present detailed conditions for iodination and purification of labeled prolactin and the optimal conditions for the assay. By the method, we found values (microgram/liter) as follows for serum prolactin: normal men. 8.9 +/- 5.2 (mea...
متن کاملRadioimmunoassay for luteinizing hormone releasing hormone in plasma.
A sensitive and specific double antibody radioimmunoassay has been developed capable of measuring LH-RH in extracted human plasma. Thyrotropin releasing hormone, lysine vasopressin and most of LH-RH analogues did not appear to affect the assay. Hypothalamic extract and some of the LH-RH analogues produced displacement curves which were parallel to that obtained with the synthetic LH-RH. Sensiti...
متن کاملRadioimmunoassay of parathyroid hormone in human plasma and tissue using commercially available antiserum and modified iodination.
The human PTH radioimmunoassay has been developed by use of bovine PTH and anti-bovine PTH antiserum, both of which were commercially available. The iodination process was modified as to use acid solution resulting in the more stable 125I-PTH. However, the PTH radioimmunoassay employed this antiserum with 125I-bovine PTH revealed that the N-terminal portion of PTH molecule was reactive only sli...
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ژورنال
عنوان ژورنال: Acta Veterinaria Scandinavica
سال: 1988
ISSN: 1751-0147
DOI: 10.1186/bf03548393